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Autoimmune and Inflammation Markers

Autoimmune and inflammation biomarkers from BBI 

BBI offers an extensive range of inflammation biomarker and autoimmune products derived either from native human tissue sources or using carefully chosen recombinant expression systems. All source materials are screened for safety using the latest sensitive methods. Products are made to order or available from stock using processes defined by specific batch records.  

We endeavour to always supply material to our customers’ timelines but rely on our clients to provide forecasts and estimates of usage.  Materials are prepared using a range of protein expression strategies and purification technologies, some unique to BBI. 

Products are mainly available as highly purified preparations,  are formulated to optimise shelf-life and provide material in the most convenient storage format. Products are often tested with or referenced against specific stored patient samples to ensure the performance. 

Please contact our Customer Services Team directly to disuss your requirements on tel:+44 (0) 1495 363000 or alternatively you can submit an enquiry here.

 

Disease Area Product code Product Quantity
Systemic Autoimmue Rheumatic Diseases

30300
30301

DFS70

0.1 mg
1.0 mg
Mixed Connective Tissue Disease / SLE 13000
13001

U1-snRNP 68/70 kDa

0.1 mg
1.0 mg
13100
13101
U1-snRNP A 0.1 mg
1.0 mg
13200
13201
U1-snRNP C 0.1 mg
1.0 mg
13300
13301
U-snRNP B/B‘ 0.1 mg
1.0 mg
Systemic Lupus Erythematosus (SLE) 17500
17501
Sm (non recombinant; bovine) 0.1 mg
1.0 mg
11600
11601
RNP/Sm (non recombinant; bovine) 0.1 mg
1.0 mg
11700
11701
SmD 0.1 mg
1.0 mg
11800
11801
SmD1 0.1 mg
1.0 mg
11900
11901
SmD2 0.1 mg
1.0 mg
12000
12001
SmD3 0.1 mg
1.0 mg
31000
31001
Nucleosome (non recombinant; bovine) 0.1 mg
1.0 mg
31100
31101
Histone (non recombinant; bovine) 0.1 mg
1.0 mg
12300
12301
dsDNA (plasmid) 0.1 mg
1.0 mg
14100
14101
Ribosomal Phosphoprotein P0 0.1 mg
1.0 mg
14200
14201
Ribosomal Phosphoprotein P1 0.1 mg
1.0 mg
14300
14301
Ribosomal Phosphoprotein P2 0.1 mg
1.0 mg
15400
15401
Proliferating Cell Nuclear Antigen (PCNA) 0.1 mg
1.0 mg
17300
17301
Ku (p70/p80) 0.1 mg
1.0 mg
19700
19701
Nucleolin 0.1 mg
1.0 mg
Sjögren's Syndrome / SLE 17400
17401
Ro/SS-A (60 kDa; recombinant) 0.1 mg
1.0 mg
15500
15501
Ro/SS-A (60 kDa; non recombinant; bovine) 0.1 mg
1.0 mg
12700
12701
Ro/SS-A (52 kDa) 0.1 mg
1.0 mg
12800
12801
La/SS-B 0.1 mg
1.0 mg
Systemic Sclerosis / CREST Syndrome 12500
12501
Centromere Protein B (CENP-B) 0.1 mg
1.0 mg
16900
16901
Centromere Protein A (CENP-A) 0.1 mg
1.0 mg
12400
12401
DNA Topoisomerase I (Scl-70; full length) 0.1 mg
1.0 mg
14500
14501
DNA Topoisomerase I (Scl-70; truncated) 0.1 mg
1.0 mg
11500
11501
DNA Topoisomerase I (Scl-70; non recombinant; bovine) 0.1 mg
1.0 mg
16000
16001
PM/Scl 100 0.1 mg
1.0 mg
17000
17001
PM/Scl 75 0.1 mg
1.0 mg
Myositis 12900
12901
Histidyl-tRNA Synthetase (Jo-1) 0.1 mg
1.0 mg
15600
15601
Threonyl-tRNA Synthetase (PL-7) 0.1 mg
1.0 mg
15700
15701
Alanyl-tRNA Synthetase (PL-12) 0.1 mg
1.0 mg
11100
11101
Glycyl-tRNA Synthetase (EJ) 0.1 mg
1.0 mg
30100
30101
Asparaginyl-tRNA Synthetase (KS) 0.1 mg
1.0 mg
18400
18401
SRP54 0.1 mg
1.0 mg
16000
16001
PM/Scl 100 0.1 mg
1.0 mg
17000
17001
PM/Scl 75 0.1 mg
1.0 mg
18100
18101
Mi-2 0.1 mg
1.0 mg
11000
11001
TIF1 gamma 0.1 mg
1.0 mg
31700
31701
NXP2 0.1 mg
1.0 mg
31600
31601
SAE1/SAE2 0.1 mg
1.0 mg
30000
30001
MDA5 0.1 mg
1.0 mg
Autoimmune Thyroid Diseases 12100
12101
Thyroid Peroxidase (TPO) 0.1 mg
1.0 mg
12200
12201
Thyroglobulin (non recombinant) 0.1 mg
1.0 mg
Celiac Disease 15200
15201
Tissue Transglutaminase (tTG; expressed in Baculovirus/Sf9) 0.1 mg
1.0 mg
14400
14401
Tissue Transglutaminase (tTG; expressed in E. coli) 0.1 mg
1.0 mg
19500
19501
Gliadin (recombinant; deamidated) 0.1 mg
1.0 mg
31500
31501
Gliadin (non recombinant) 0.1 mg
1.0 mg
Autoimmune Liver Diseases 31800
31801
Cytochrome P450 2D6 (LKM 1; ng) 0.1 mg
1.0 mg
13700
13701
Formiminotransferase Cyclodeaminase (LC 1) 0.1 mg
1.0 mg
30800
30801
SLA/LP 0.1 mg
1.0 mg
18000
18001
M2 0.1 mg
1.0 mg
17700
17701
BCOADC-E2 0.1 mg
1.0 mg
17800
17801
OGDC-E2 0.1 mg
1.0 mg
17900
17901
PDC-E2 0.1 mg
1.0 mg
18900
18901
Sp100 0.1 mg
1.0 mg
19000
19001
gp210 0.1 mg
1.0 mg
19400
19401
Nup62 0.1 mg
1.0 mg
ANCA associated Diseases 18500
18501
Myeloperoxidase (MPO; non recombinant) 0.1 mg
1.0 mg
18600
18601
Proteinase 3 (PR3; non recombinant) 0.1 mg
1.0 mg
19200
19201
BPI (non recombinant) 0.1 mg
1.0 mg
Antiphospholipid Syndrome / Thromboembolic Syndrome 14900
14901
ß2-Glycoprotein 1 (Apolipoprotein H) 0.1 mg
1.0 mg
11300
11301
ß2-Glycoprotein 1 (Apo H; non recombinant; human) 0.1 mg
1.0 mg
11400
11401
ß2-Glycoprotein 1 (Apo H; non recombinant; bovine) 0.1 mg
1.0 mg
Anti-GBM Disease 16800
16801
Glomerular Basement Membrane (GBM; dissociated) 0.1 mg
1.0 mg
Crohn's Disease 19600
19601
GP2 0.1 mg
1.0 mg
Diabetes Mellitus 31900
31901
Glutamate Decarboxylase 65 kDa (GAD65; ng) 0.1 mg
1.0 mg
30500
30501
IA-2 (ICA 512) 0.1 mg
1.0 mg
Pernicious Anemia 16700
16701
Intrinsic Factor 0.1 mg
1.0 mg

 

Inflammation markers

Product description Product code
Alpha-1-Antichymotrypsin P159-5
Alpha-1-antitrypsin P165-5
Complement C4c P151-8
Human C-Reactive Protein (CRP) P100-0
Human C-Reactive Protein (CRP) P100-7N
Prealbumin P171-1
Prealbumin P171-2
Recombinant Human Procalcitonin (Recombinant) P742-1

 

Antigens FAQs

Our team are here to help! If you can’t find the answer you are looking for contact us.

For our European customers please contact your BBI sales representative or reach out to our customer services team. For our US and Asia customers you can also contact your BBI representative for support however sales enquiries, where relevant, will be routed through a local representative. In the US for DIARECT catalogue products, these should be ordered from our US distribution partner Surmodics.

Upon receipt, our antigens / antibodies should be stored frozen at - 70°C or below until use. We recommend thawing at room temperature, but thawed products should not remain at room temperature for extended periods of time. Mix products gently before use. If you plan on using only a portion of the product, please thaw and dispense it into aliquot volumes that are most practical for your application and store frozen at - 70°C or below. Please note that we cannot guarantee the shelf life after the antigens / antibodies have been re-dispensed and adviceagainst repeated freeze thaw cycles.

While general recommendations for antigen / antibody storage conditions and buffers are given in the antigen's / antibody's specification sheet, which can be found in the “+INFO” section associated with each antigen / antibody on DIARECT's website, the detailed buffer composition is available together with the lot-specific Certificate of Analysis (CoA).

No, we do not recommend using antigens / antibodies without a guaranteed cold chain. If you receive a thawed product please inform our customer service immediately. Regarding dry ice shipments please visit section "shipping information" on our website for detailed information on temperature
tests.

For each antigen / antibody, DIARECT created a “+INFO” section on its website, which contains the antigen's / antibody's material safety data sheet (MSDS) and specification sheet. The latter provides general recommendations regarding antigen / antibody storage and buffers. For select antigens / antibodies, the “+INFO” section also contains a brochure offering some background information and immunological test data. You can also contact your local representative or info-dia@bbisolutions.com for more details.

Most of our antigens have a guaranteed shelf life of five years unless otherwise noted on our Certificates of Analysis (CoA) and data sheets, and providing that they are kept frozen at -70°C or below. For the shelf life of our antibodies, please check the expiry date on the lot-specific CoA provided with the product.

We guarantee the reservation of a specified amount of antigen(s). Typically, customers reserve the amount of antigen(s) needed for a manufacturing period of up to 18 months. You simply inform us of
your antigen requirements for this period, and every 4 months you will receive an updated overview regarding the accuracy of your estimate. The reservation system also includes the allocation of early
enough samples of new lots of the respective antigen(s). This enables the customer to evaluate the new lot at an early stage for future reservations and allows a smooth transition to this new lot.
Advantages provided by DIARECT's reservation system:
- Security of supply with no additional costs
- No need for continual readjustment and fine tuning of your assay because of new antigen lots

BV and Ec represent the type of expression system that was used to produce the antigen of interest. BV stands for DIARECT's baculovirus/insect cell expression system, while Ec stands for DIARECT's E. coli expression system.

For purification, the preferred technique is the very effective, yet gentle immobilized metal-ion affinity chromatography (IMAC) in combination with His-tag technology, which yields physiologically and
immunologically active proteins. Our native proteins are purified using appropriate standard chromatography purification methods including ion exchange, size exclusion and immunoaffinity.

DIARECT's recombinant and native antigens / antibodies - always produced according to documented procedures (SOPs) and manufactured per EN ISO 13485 and 9001 standards - undergoing a rigorous series of tests in our quality control laboratory. Prior to its approval and release, each lot is subjected to various biochemical and immunological analyses to assure conformance to our strict product specifications. The goal of these extensive inspections is to ensure lot-to-lot conformance, which guarantees consistent results.

General information regarding the origin and expression constructs of antigens are provided in the respective specification sheets, however, exact sequence data are considered proprietary information. Most recombinant antigen preparations are supplied with a 6xHis-tag as part of the sequence.

DIARECT antigens are manufactured in our EN ISO 13485 and EN ISO 9001 audited facility in Freiburg, Germany. Supply is either direct from our Freiburg facility or may be managed by our local representatives in specific territories.

Why choose Autoimmune antigens from BBI Solutions? 

BBI and our scientists in Freiburg are world renowned for providing a comprehensive and high-quality range of autoimmune antigens. Many of these antigens are reference materials for some rare and important disease assays. 

Our autoimmune antigens are used in many primary screening methods for determination of the specific target for a serological autoimmune response. The company objective has been to derive an extensive list of targets for specific disease states where high specification and highly reproducible products are required to give the best performance across different assay platforms including ELISA, microarray and line assay.  

In addition, BBI supply a comprehensive range of serum biomarker indicators of inflammation such as CRP and Procalcitonin. These materials are routinely used as critical reagents to prepare IVD assay standards and quality control materials. BBI derives its range of autoimmune antigens by molecular methods and the proteins are expressed in specific host organisms. The host cell lines are stored and propagated in our bioreactor systems. Some of our recombinant products  are expressed with specific His-tag sequences to facilitate purification by nickel chelate affinity chromatography. 

Some of our inflammation biomarkers are isolated as native products from human plasma or tissue sources. All human derived source material is always screened for viral safety for markers such as HIV, Hepatitis B and C prior to use. These native and untagged products are purified by a sequential series of different chromatography techniques. The purified proteins are then formulated liquid or lyophilised in the most appropriate stabilising buffer and filtered to 0.2µm to ensure low bioburden.  

Quality embedded at every step of the way 

Products are characterised prior to fill to determine the recovery and purity of the protein using a range of analytical techniques. Techniques such as Coomassie stained SDS-PAGE are used to test and verify the product purity. Importantly, our autoimmune markers for serological applications are tested against a defined panel of patient samples. The immunological recovery of our inflammatory biomarkers is tested by established immunoassay methods. 

After the products have been filled and formulated, they are retested by our independent Quality Control laboratory to verify the analytical results and define the biological integrity. Materials are stored and shipped from our Sittingbourne or Freiburg facilities.  

Materials produced at our Sittingbourne facility are shipped directly to all our customers in all territories worldwide. 

Antigens prepared at our Freiburg facility  are supplied directly to clients in Europe and non-China Asia territories.   

For customers in America and China, our Freiburg antigens are distributed through our carefully selected and highly reputable partners. 

Technical Documents

If you require any further product specific technical information or datasheets please submit your request to our technical team here 

Last updated: 25/06/2021

Clinical Chemistry Brochure

Last updated: 16/03/2022

CENP Antigens Brochure

Last updated: 16/03/2022

DFS70 Antigens Brochure

Last updated: 16/03/2022

Diabetes Antigens Brochure

Last updated: 16/03/2022

Glycoprotein 2 Antigen Brochure

Last updated: 16/03/2022

Histone, Nucleosome, dsDNA Antigens Brochure

Last updated: 16/03/2022

Intrinisc Factor Antigen Brochure

Last updated: 16/03/2022

MPO, PR3, BPI Antigens Brochure

Last updated: 16/03/2022

Myositis Antigens Brochure

Last updated: 16/03/2022

Nucleolin Antigens Brochure

Last updated: 16/03/2022

Ribosomal Phosphoproteins Brochure

Last updated: 16/03/2022

Primary Biliary Cirrhosis Antigens Brochure

Last updated: 25/06/2021

Clinical Chemistry Brochure

Last updated: 16/03/2022

CENP Antigens Brochure

Last updated: 16/03/2022

DFS70 Antigens Brochure

Last updated: 16/03/2022

Diabetes Antigens Brochure

Last updated: 16/03/2022

Glycoprotein 2 Antigen Brochure

Last updated: 16/03/2022

Histone, Nucleosome, dsDNA Antigens Brochure

Last updated: 16/03/2022

Intrinisc Factor Antigen Brochure

Last updated: 16/03/2022

MPO, PR3, BPI Antigens Brochure

Last updated: 16/03/2022

Myositis Antigens Brochure

Last updated: 16/03/2022

Nucleolin Antigens Brochure

Last updated: 16/03/2022

Ribosomal Phosphoproteins Brochure

Last updated: 16/03/2022

Primary Biliary Cirrhosis Antigens Brochure

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