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Secondary Antibodies and Controls

Why Choose BBI Solutions Monoclonal Antibodies?

At BBI Solutions, we offer a diverse range of monoclonal antibodies to ensure the reliability and performance of your diagnostic assays. 

Specialised subset of 10 monoclonal anti-human secondary antibodies for broad recognition of human immunoglobulins.   Control antibodies for the detection of Histidine-tagged and Pegylated proteins with some of the most cost-effective products in the marketplace.  Strong security of supply: BBI’s panel of anti-human secondary antibodies, anti-His tag antibody, and anti-PEG antibodies are manufactured and controlled by BBI Solutions at our cGMP* facility in Maine, USA  

Trusted by assay developers wordwide

BBI Solutions’ monoclonal antibodies are renowned as sensitive, cost-efficient products for assay developers, manufacturers, and researchers worldwide. They are available in both small and bulk quantities depending on your individual needs. 

Please contact our Customer Services Team directly to discuss your requirements on tel:+44 (0) 1495 363000 or alternatively you can submit an enquiry here.

*21 CFR Part 820, For research and In Vitro diagnostic use.

Related Products

If you require any further product specific technical information or datasheets please submit your request to our technical team here 

Antibodies FAQs

Our team are here to help! If you can’t find the answer you are looking for contact us.

Antibodies are a critical component of the defense arsenal assisting with identifying, capturing, and removing potential threats as well as protecting against future invasions. Antibodies are gammaglobulin proteins, predominantly referred to as immunoglobulins (Ig). A monomeric antibody is composed of two heavy chains and two light chains covalently linked together through disulfide bonds to generate a Y-shaped structure. This Y-shaped structure provides a bifunctional capacity: 1) antigen-binding through the Fab (antigen binding fragment), and 2) interaction with immune cells and proteins (fragment crystallizable or Fc) to initiate and regulate host defence mechanisms.

Polyclonal antibodies refer to a mixture of immunoglobulin molecules that are secreted against a particular antigen. They interact with different epitopes on the same antigen and their manufacture does not require the use of hydbridomas. Monoclonal antibodies refer to a homogenous population of antibodies that are produced by a single clone of plasma B cells. They interact with a particular epitope on the antigen and their
manufacture requires the use of a hybridoma cell line.

This varies between products, please consult the example Certificate of Analysis (found in the ‘Technical Documents’ section of the product page) for this information.

This varies between products, please consult the example Certificate of Analysis (found in the ‘Technical Documents’ section of the product page) for this information.

Yes, we manufacture many of our own products at our 2 manufacturing sites in Maine, USA and Porton Down, UK. All sites are ISO13485 certified.

BBI Solutions offers a growing portfolio of performance proven antibodies. With our in-house custom antibody expertise, customers can rely on BBI to be a trusted partner in antibody support, from secure supply, to matched pair recommendations, and trouble shooting performance. Custom manufacturing lots and conjugation services are also value-added services available for most of our antibodies to suit your assay development needs.

A matched antibody pair consists of two antibodies, a capture and a detection antibody. In a matched pair, each antibody is specific for a different and non-interfering epitope of the antigen.


Rabbit, Goat and Sheep.

Roller bottle for lot sizes up to 1gram. Hollow fibre continuous perfusion system for lot sizes greater than 1 gram.

4-6 months for a monoclonal antibody and 3-4 months for a polyclonal.

A discussion with a service team member will be necessary regarding this, however monoclonal development projects start at $20k and up.

As this is a complex subject, a separate FAQ has been written for it and can be found on the Antibody Development services page.

The function of Secondary Antibodies 

Secondary antibodies are a common and critical component of immunoassays. Just as human immunoglobulin (IgA, IgG, IgE, & IgM) have diverse functions within the body, detecting human immunoglobulin has varied applications in the diagnosis and management of human disease including:

  • Serology based diagnostic testing
  •  Immunohaematology 
  •  Autoimmune function testing
  •  Quantitative immunoglobulins test
  • IgG subclass-specific quantitation 

Why choose BBI Secondary Antibodies?  

  • Specialised subset of secondary antibodies for broad recognition of immunoglobulins  
  • Security of Supply: BBI’s panel of 10 monoclonal anti-human secondary antibodies is manufactured and controlled by BBI at our cGMP* facility in Maine, USA + 
  • Need for discriminating testing and use of multiplexed assays are benefited by monoclonal antibodies rather than polyclonal 

Control Antibodies 

Anti-His Tag 

BBI’s anti-His tag antibody is: 

  • Highly sensitive with strong affinity 
  • Specific – no detection of E.coli host cell proteins 
  • Recognizes polyhistidine tags of 4X or greater engineered at either the N- or C-terminus 


BBI’s anti-PEG antibodies are: 

  • Specific – recognize both linear and branched PEG 
  • Recognizes both conjugated and free forms of PEG 

*21 CFR Part 820, For research and In Vitro diagnostic use

Technical Documents

If you require any further product specific technical information or datasheets please submit your request to our technical team here 

Last updated: 26/03/2021

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The acquisition of DIARECT in June 2020 and their world leading recombinant autoimmune antigen capability immediately enhances BBI’s portfolio and position as a ‘complete’ immunoassay reagent supplier. This further enhances BBI’s position as the world’s largest diagnostics components company with a market leading antigen portfolio.


Maine Biotechnology Services brings 27 years of knowledge, experience, process development, and project management to our customers to ensure the best possible antibodies for any application. As antibody development goals in the biotechnology and pharmaceutical industry have evolved the acquisition of MBS will help BBI further meet and exceed our customers needs.


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