What is pernicious anaemia? Intrinsic Factor is primarily used in the diagnosis of pernicious anaemia, a condition caused by a vitamin B12 deficiency. Prevalence in the general population varies from around 3% - 5%, and increases dramatically to 5% - 20% among people older than 65 years. The role of Intrinsic Factor in diagnosis Intrinsic Factor has a high affinity and specificity to vitamin B12, which makes it ideal for use in immunoassays. Most commercial assays utilise a competitive binding technique generating a chemiluminescent signal. Commercial assays can usually detect to a level of ~50 pg/ml vitamin B12. Why choose BBI for your supply? BBI is the only commercial manufacturer of high purity Intrinsic Factor. BBI’s porcine Intrinsic Factor is manufactured in the UK, under a quality system compliant with ISO 13485:2016. Using our expertise in raw material sourcing and protein purification we achieve exceptional purity, making BBI the supplier of choice.