ALPIXG-Alkaline phosphatase

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ALPIXG-Alkaline phosphatase

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#SKU ALPIXG

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Product Details

Alkaline Phosphatase (ALP) is commonly used as a label in immunoassays such as ELISA, and in blotting and histochemistry. Once conjugated to antibodies, antigens, or streptavidin, its low backgrounds and linear reaction rate enable increased sensitivity over extended incubation times. It can be used with a variety of substrates producing precipitated or soluble chromogens, or with chemiluminescent and fluorogenic substrates for enhanced sensitivity. BBI Solutions (BBI) is the world’s leading producer of bovine ALP and has supplied the diagnostics industry with the widest range and highest specific activity material for over 25 years. Our raw materials selection, harvesting protocol and production procedures have been optimised to ensure that we achieve the highest possible specific activity and batch-to-batch consistency. We have also developed a series of preparations to provide a range of specific activities to suit a wide range of applications. Our many commercial partners have come to rely on ALP from BBI for proven performance in their immunoassay systems.

ALPIXG

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Attributes

Description

  • Abbreviations
  • ALP, Alk. Phos
  • Associated Activity
  • Bovine IgG: Not detectableChromatogram analysis: Greater than 90% pure by molecular exclusion chromatographyProtein Concentration: 30-40 mg/ml (Determined by Biuret procedure.)Available amino groups: 8 - 13 moles amino groups per mole enzymeCarbohydrate content: 4.0 – 6.5%
  • Lead Time
  • 8 weeks
  • Storage
  • Store at 2°C to 8°C. DO NOT FREEZE.
  • Shelf Life
  • 10 years
  • Source
  • Bovine Intestinal mucosa
  • Activity
  • 1,000 Glycine U/mg protein (Equivalent to approximately 3,000 DEA U/mg protein)
  • EC Number
  • 3.1.3.1
  • EINECS
  • 232-631-4
  • Systematic Name
  • Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
  • Unit Definition
  • Glycine: That amount of enzyme causing the hydrolysis of one micromole of p-nitrophenyl phosphate per minute at 25C and pH 9.6 (glycine buffer). DEA: That amount of enzyme causing the hydrolysis of one micromole of p-nitrophenyl phosphate per minute at 37C and pH 9.8 (diethanolamine buffer).

Technical Documents

Product Specification (540.62KB)

Analytical Procedures (140.23KB)

Product Information (513.63KB)

Method Analytical Procedure (131.28KB)

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