ALPI12G - Alkaline Phosphatase

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ALPI12G - Alkaline Phosphatase

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Product Details

Alkaline Phosphatase (ALP) is commonly used as a label in immunoassays such as ELISA, and in blotting and histochemistry. Once conjugated to antibodies, antigens, or streptavidin, its low backgrounds and linear reaction rate enable increased sensitivity over extended incubation times. It can be used with a variety of substrates producing precipitated or soluble chromogens, or with chemiluminescent and fluorogenic substrates for enhanced sensitivity. BBI Solutions (BBI) is the world’s leading producer of bovine ALP and has supplied the diagnostics industry with the widest range and highest specific activity material for over 25 years. Our raw materials selection, harvesting protocol and production procedures have been optimised to ensure that we achieve the highest possible specific activity and batch-to-batch consistency. We have also developed a series of preparations to provide a range of specific activities to suit a wide range of applications. Our many commercial partners have come to rely on ALP from BBI for proven performance in their immunoassay systems.


More information



  • Abbreviations
  • ALP, Alk. Phos
  • Associated Activity
  • Bovine IgG: Not detectableChromatogram analysis: Greater than 90% pure by molecular exclusion chromatographyProtein Concentration: 10-20 mg/ml (Determined by Biuret procedure) Available amino groups: 8 - 13 moles amino groups per mole enzyme Carbohydrate content: 4.0 – 6.5%
  • Recommended Use
  • Recommended as an enzyme label in elisa assay, blotting and histochemistry.
  • Lead Time
  • 8 weeks
  • Storage
  • Store at 2°C to 8°C. DO NOT FREEZE.
  • Purity/grade
  • > 1600 Glycine U/mg
  • Source
  • Bovine Intestinal mucosa
  • Activity
  • ≥2000 Glycine U/mg protein, (Equivalent to approximately 6000 DEA U/mg protein).
  • EC Number
  • 232-631-4
  • Systematic Name
  • Orthophosphoric-monoester phosphohydrolase (alkaline optimum).

Technical Documents

Product Specification (106.43KB)

Analytical Procedures (140.23KB)

Product Information (513.63KB)

Method Analytical Procedure (0KB)

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BBI Solutions Brands


The acquisition of DIARECT in June 2020 and their world leading recombinant autoimmune antigen capability immediately enhances BBI’s portfolio and position as a ‘complete’ immunoassay reagent supplier. This further enhances BBI’s position as the world’s largest diagnostics components company with a market leading antigen portfolio.


Maine Biotechnology Services brings 27 years of knowledge, experience, process development, and project management to our customers to ensure the best possible antibodies for any application. As antibody development goals in the biotechnology and pharmaceutical industry have evolved the acquisition of MBS will help BBI further meet and exceed our customers needs.


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