Research
Enzymes for Research
BBI provide a number of enzymes for research applications. These are available from sample to bulk sizes to suit both small and large scale requirements
Our refined sourcing and manufacturing methods ensure high-purity, stable, and consistent Chymotrypsin, Trypsin, and Hyaluronidase products for robust performance in clinical and research environments.
| Product Name | Code | Type | Applications | |
|---|---|---|---|---|
| Ribonuclease ≥70U/mg material | RN1 | Enzymes | For Plasmid purification procedures and for digestion of RNA | Buy now |
| Ribonuclease ≥70U/mg material | RN2 | Enzymes | For Plasmid purification procedures and for digestion of RNA | Buy now |
| Ribonuclease ≥81U/mg material | RN3 | Enzymes | For Plasmid purification procedures and for digestion of RNA | Buy now |
Brochure
Products & Services
Inside this catalog you’ll find one of the industry’s broadest collections of enzymes, antibodies, antigens, and more – meticulously developed to power world-class diagnostics and keep your pipeline moving.
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FAQs
What purity/activity levels can I expect from horseradish peroxidase?
There are options for multiple grades, including those that deliver ≥ 90 % isozyme C and ~ 280 U mg-¹ activity for clean, rapid conjugation.
Do you supply bulk volumes?
Yes – order anywhere from R&D vials to > 10 000 ku ALP or > 1 000 MU GOx; we pack to order.
What documents ship with each batch?
We operate an ISO13485 quality system. That means a Certificate of Analysis and Safety Data Sheet are available to you.
Where are your enzymes manufactured?
All core enzymes are produced in our ISO 13485-certified plants in Crumlin (UK) and Cape Town (SA).
How should I store and ship each enzyme?
Different enzymes have different storage and shipping requirements. Check the individual enzymes documentation for exact details. On arrival store ALP at 2–8 °C glycerol solution. Lyophilised GOx, HRP, RNase and Urease keep best desiccated below –15 °C.
Can I extend the best-before date on my enzyme?
Email technicalsupport@bbisolutions.com—many grades qualify for a validated shelf-life extension.
Which applications are BBI enzymes commonly used in?
Biosensors, immunoassays, clinical chemistry and molecular diagnostics are the four most common uses.
What makes your alkaline phosphatase a good ELISA label?
Highest-activity conjugation grade (> 2 000 Gly U mg-¹) with < 10 % activity loss over 48 months – delivering low background and extended incubations.
Request a sample
Chymotrypsin
The chymotrypsins, of which there are several, are serine proteases derived from the inactive precursor Chymotrypsinogen-A (Alpha– Chymotrypsinogen) and B, which are present in the pancreas and pancreatic juice. Chymotrypsinogen-A is activated by Trypsin, and depending on the method of activation, the principle chymotrypsins may be obtained.
These are referred to as Alpha, beta, gamma, delta and pi chymotrypsins.
Products
| Product Name | Code | Type | Applications | |
|---|---|---|---|---|
| Chymotrypsin >5.0 µKatal/mg | 031110BBI | Buy now |
Trypsin
Trypsin is widely distributed in animals and in some bacteria and is classed as a serine protease. Trypsin is present in the pancreas and pancreatic juice in vertebrates. Trypsinogen, secreted into the duodenum as a pro-enzyme, is activated by Enterokinase secreted by the duodenal mucosa. Trypsin is obtained by activation of its inactive precursor (Trypsinogen) by Enterokinase and by Trypsin itself. Calcium stimulates the autoactivation of Trypsinogen.
Trypsin has a high degree of substrate specificity as it catalyses the hydrolysis of peptide bonds on the carboxyl terminus of positively charged amino acids such as Lysine and Arginine. Trypsin has a weak action on native proteins and will not hydrolyze serum globulins, haemoglobin, ovalbumin or collagen
Hyaluronidase
Testicular hyaluronidase is a typical glucosidase having both endohexosaminidase and transglycosidase activity.
Substrates are hyaluronic acid and also chondroitin sulphate A and C. Products of hydrolysis are a series of oligosaccharides, mainly tetrasaccharides. Hyaluronidase is often used in conjunction with collagenase to
dissociate the extracellular matrix between cells of animal tissue, in order to release viable cells for use in tissue culture. It may also be used to clarify synovial fluids in order to make cell counts possible.
Products
| Product Name | Code | Type | Applications | |
|---|---|---|---|---|
| Hyaluronidase >1000 U/mg Source : Bovine testes | 081246BBI | Buy now | ||
| Hyaluronidase >350 U/mg Source : Bovine testes | 081245BBI | Buy now | ||
| Hyaluronidase >500 U/mg Source : Bovine testes | 081240BBI | Buy now | ||
| Hyaluronidase cGMP >400 U/mg Source : Ovine testes | 081239H03 | Buy now |
Deoxyribonuclease
Deoxyribonuclease (DNase) occurs predominantly in the pancreas, but has also been found in other tissues and in the salivary glands. DNase is an endonuclease enzyme that hydrolyses phosphodiester
bonds adjacent to pyrimidine nucleotides of deoxyribonucleic acid (DNA), yielding 5’-phosphate terminated polynucleotides with a free hydroxyl group at the 3’ position.
Products
| Product Name | Code | Type | Applications | |
|---|---|---|---|---|
| Deoxyribonuclease >2500 Kunitz Units/mg | 041160BBI | Buy now |
Confidence
Be confident in supply sustainability by working directly with the manufacturer.
Specific & Flexible
Get a product which meets your exact specifications with our flexible approach.
Cost
Save on cost by ordering in bulk.
Performance
Be confident in product performance with access to our regular benchmarking analysis.
65 years of enzyme expertise
Our enzymes have been used by the diagnostics industry for over 65 years, within a wide range of applications, including:
- Biosensors
- Immunoassays
- Clinical chemistry
- Pharmaceutical
- Molecular
- Research
BBI have developed effective, reliable and high-quality products that are used by industry leaders across the world. With international multi-site manufacturing facilities and worldwide distribution capabilities supplying a truly global and diverse customer base, BBI is the partner of choice for your reagents.
Core Competencies
Our product range includes enzymes for biosensor, immunoassay, clinical chemistry, molecular and pharmaceutical applications with a range of different grades to suit all applications.
We aim to develop, manufacture and supply high quality enzymes which meet both customer and regulatory requirements.
We use quality raw materials, innovative production methods and product formulations to achieve maximum stability for our products. We test our products regularly to ensure this remains consistent.
If one of our off-the-shelf products does not meet your requirements, we can offer custom development and manufacturing services to deliver an enzyme to your specification, perfect for your application.
BBI's Capabilities
Specialists
We have extensive experience in sourcing raw materials worldwide
Salt/solvent fractionation
Batch sizes of up to 5,000 litres
Chromatography
Ion exchange chromatography, affinity chromatography, size exclusion chromatography, hydrophobic interaction chromatography
Crystallisation
Highly effective protein purification using solvent and aqueous media
Ultrafiltration
Volumes of 2,500 - 30,000 litres
Freeze drying
Capability of 49 - 420 litres per cycle
Quality control
In-process analysis and monitoring, development of assay/test procedures
Product handling/sampling
We offer special packing according to customer specifications