RN3-Ribonuclease

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RN3-Ribonuclease

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#SKU RN3

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Product Details

Ribonuclease (RNase) is classified as an endonuclease, which specifically cleaves phosphodiester bonds at the 3’-end of pyrimidine nucleosides and at the 5’-ribose of a nucleotide, ribonucleic acid (RNA). Ribonuclease can be used in the following applications: + In isolation of DNA for RNA-free DNA, especially plasmid DNA + Structural studies of RNA + The depolymerisation of viscous biological suspensions, rendering RNA-free solutions
RN3

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Attributes

Description

  • Abbreviations
  • Rnase
  • Associated Activity
  • DNase contamination: There is no change to the binding pattern of Hind III-digested λDNA following incubation of 1µg with 0.5U of RN3 for 30minutes at 37°C.
  • Lead Time
  • 6 weeks
  • Storage
  • Store desiccated at -15°C or below. Allow to come
  • Shelf Life
  • 10 years
  • Source
  • Bovine pancreas
  • Activity
  • ≥81U/mg material RNase A content greater than 95% by ion exchange chromatography.
  • EC Number
  • 3.1.27.5
  • Solubility
  • Dissolves readily at 2mg/ml in Analytical grade water to give a clear solution.
  • Systematic Name
  • Ribonucleate 3’-pyrimidino-oligonucleotideohydrolase
  • Unit Definition
  • That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0

Technical Documents

Product Specification (126.52KB)

Analytical Procedures (136.03KB)

Product Information (352.7KB)

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