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Our scientists perform more than 500 individual conjugations every year for a variety of unique applications

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Conjugation

One of the most important factors in the development of a reliable assay is the quality of the gold conjugate. At BBI Solutions we use the highest quality grades of gold nanoparticles and apply our unique conjugation protocol to deliver an optimised product that meets the demands of your assay.

Whether you are looking to bind gold to a specific antibody, and need a custom conjugation, or off the shelf products, BBI can help and deliver.

Custom Conjugation Service

BBI’s custom conjugation service allows us to develop high quality conjugates using reagents provided by you or sourced by us.

Our service includes:

Advice on the optimum nanoparticle and size to use for your application Conjugation of your antibody or protein to a nanoparticle and size of your choice
Particle Analysis Batch sizes up to 24L at OD10
Sourcing your required antibody or protein Long term stability analysis (on request)
Selection of appropriate final buffer for the conjugate Custom OD preparation (not limited to OD10)
Antibody Screening QC test development
Optimisation of sensitivity and potential stability Comprehensive lab report on conjugate performance

Designed to meet your individual needs

Specificity, sensitivity and reproducibility should define the specification and fulfil your absolute minimum expectations of the conjugate.

Our scientists work in partnership with customers to bring all the vital ingredients together and provide sensitive, stable, reproducible conjugates time after time.

At the start of the project we will ask you for the following customer inputs and deliver the listed outputs:

Customer inputs

BBI outputs

Choose your target protein (antibody/protein) Initial 10ml of final optimised product – (enough for 1000 prototype lateral flow strips*)
Identify target sensitivity Excess reagents banked for future production
Identify particle size Full laboratory report on performance customised to your requirements
Concentration of final conjugate required Access to technical team post conjugation
Provide 3-5mg of protein for conjugation and QC materials *using a 1:5 dilution of OD10 conjugate per strip

A quality solution

quality pic

Every custom conjugation is QC tested with customer supplied materials. Your unique sign-off procedure is brought into BBI to ensure every batch of gold we deliver meets your requirements and works with your in-house system.

Bulk production

Following collaboration on the optimisation of the conjugate to test specifications, we are able to provide conjugate in bulk. We can put a commercial supply plan in place and supply single batch volumes of up to 24L at OD10, delivered on a two week turnaround.

To find out more about our conjugation service, contact us »

Off the shelf conjugates

For optimal performance, cost effectiveness and guaranteed quality, we offer off the shelf catalogue gold conjugates, designed to help you develop and manufacture your assay at a rapid and economical rate.

Our catalogue range includes:

• Anti-biotin • Anti-human • Control line

• Anti-species • Disease state conjugates • Fertility conjugates

All conjugates manufactured using our unique gold nanoparticles, providing outstanding performance and long term stability.

Click the product code in the table below to purchase or find out more

If you are looking for a conjugate which is not in our standard product range, take a look at our custom conjugation service »

Anti-Biotin

Anti-Human

Control line

Anti-species

Disease state

Fertility

Monoclonal anti Biotin 40nm

Goat anti Human IgG (H+L) 40nm

Chicken IgY 40nm

Goat anti Rabbit 40nm

Monoclonal anti Hepatitis 40nm

Human Chorionic Gonadtrophin (HCG) 40nm

Goat anti Biotin 40nm

Goat anti Human IgG 40nm

Mouse IgG 40nm

Goat anti Mouse IgG (H+L) 40nm

Human Lutienizing Hormone (LH) 40nm

Goat anti Biotin 20nm

Goat anti Human IgM 40nm

Bovine Serum Albumin 40nm *

Streptavidin 40nm

Goat anti Human IgA 40nm

Goat anti Mouse IgG (H+L) 40nm

Streptavidin 20nm

Protein A 40nm

Goat anti Rabbit 40nm

Protein A 20nm

*Negative control conjugate

Gold Conjugates for Microscopy

Gold labels offer an effective method of labelling antigens for visualisation in the electron microscope. BBI has a range of EM grade conjugates that combine different antibodies on a range of particles, predominantly from 2, 5, 10, 15, 20, 30 & 40nm. For researchers beginning to work with immunogold labelling, we recommend 10nm gold conjugates for observation, at a range of magnifications.

The main advantages of gold labelling lie in the high contrast and unequivocal nature of the label, the range of particle sizes suitable for different magnifications, the extreme sensitivity and the stability of the signal.

Our technical team are available to discuss which conjugate is right for your application or to answer your nanoparticle based questions. Contact us »

Oligo Custom Conjugation Service

Oligonucleotide custom conjugation service offers high-quality conjugation of customer’s DNA and RNA oligonucleotides to BBI’s gold nanoparticle range, using our proprietary conjugation method.

Oligonucleotides are chemically synthesised to be very specific to a target sequence. They can even be synthesised to detect molecules of interest that would otherwise not be possible using antibodies, such as very small molecules, toxins and targets with low immunogenicity.

BBI’s high quality gold nanoparticles combined with our established custom conjugation methods, and the stability and reproducibility of oligonucleotides could result in a more robust conjugate for your assay.

Talk to one of our technical experts to learn more about our Oligo custom conjugation service, contact us »

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