Troponin I Antibody

Troponin I Antibody

A NEW antibody specific for cardiac troponin I is available from BBI Solutions, sold under license from bioMérieux. The antibody is from clone 3D5F7 that is used in bioMérieux’s commercial assays and is manufactured under cGMP conditions at BBI’s Maine facility.

Customers can be confident in sampling this performance tested antibody to Troponin I that has demonstrated the ability to function as a detector antibody in a sandwich immunoassay format when paired with capture antibodies specific for cTnI epitopes aa24-40 and/or aa41-49. Cardiac Troponin I is a common clinical indicator of cardiovascular disease. By manufacturing the antibody in-house, BBI can ensure secure supply with increased batch-to-batch consistency.

MAB283P tested as a detector antibody in sensitive bead-based ELISA

Beads (Captures) = MAB1* & MAB2*
Detector =MAB283P (clone 3D5F7)

  Calibrators:

cTn I-T-C

Rec cTnI

Conc. (pg/mL)

Mean AEB

Mean AEB

0.0

0.014

0.015

0.3

0.054

0.046

1.1

0.136

0.127

3.8

0.396

0.410

13.3

1.467

1.579

46.5

5.239

4.973

162.9

15.837

14.819

 

Figure 1: MAB1* & MAB2* were coated on beads and MAB283P (clone 3D5F7) used to detect cardiac Troponin I either in a complex form or as a purified recombinant protein (BBI code P725-3).

*MAB1 & MAB2 correspond to antibodies with epitopes targeting aa 24-40 and 41-49 of cTnI.

Data provided by BioCheck, Inc, and generated using Simoa HD-1 Analyser

Antibody clone (3D5F7, epitope cTnI aa 87-95) has been successfully used in commercialised bioMérieux immunoassay products* as the detector antibody

Figure 2: An optimised Fluorescence Immunoassay standard curve showing high sensitivity and prepared using cardiac TnI antibody clone 3D5F7 as a detector antibody in conjunction with other antibodies

Figure 2: An optimised Fluorescence Immunoassay standard curve showing high sensitivity and prepared using cardiac TnI antibody clone 3D5F7 as a detector antibody in conjunction with other antibodies

Note:Customers have seen excellent results pairing MAB283P with capture antibodies targeting epitopes aa 24-40 and/or aa 41-49.

*VIDAS® and/or VIKIA® and/or BIONEXIA®

Why choose BBI's Antibodies?

+ The antibody clone is used in commercialised bioMerieux assays – proven performance
+ Manufactured in-house at BBI for security of supply
+ cGMP production and quality management for increased batch-to-batch consistency
+ Suite of reagents for your troponin assay needs

Please enquire to order a sample today or to discuss bulk pricing.

Copyright © 2019 BBI Solutions. All rights reserved. Designed trademarks and brands are the property of their respective owners. BBIUS