NEW Antibodies, specific for HIV-1 p24

NEW from BBI Solutions R&D, HIV-1 p24 antibodies with multiple recommended matched pair combinations that are ready for use in ELISA or lateral flow applications. All four antibodies were developed with IVD customers in mind and are produced in our cGMP facility ensuring long-term security of supply.

HIV p24 is the structural core protein of HIV. The p24 protein, also known as the capsid protein “CA”, is heavily involved in viral infection and is an important diagnostic marker. Tests that combine the detection of p24 with serological detection of human anti-HIV-1 p24 antibodies shorten the post-infection test window to approximately 2 weeks.

Development

Recombinant HIV-1 p24 (P714-3-PROTO) was used as the immunogen to generate over 100 antibody candidates. Careful matched pair screening of monoclonal antibodies resulted in four clones with sensitive and diverse binding characteristics to both recombinant HIV-1 p24 (heat map shown below) and native HIV-1 p24.

Colorimetric sandwich ELISA utilising unconjugated capture antibody (left-hand column) paired with biotinylated detection antibody (top row). The antigen used was recombinant HIV-1 p24 at 2.5 ng/mL.

Colorimetric sandwich ELISA utilising unconjugated capture antibody (left-hand column) paired with biotinylated detection antibody (top row). The antigen used was recombinant HIV-1 p24 at 2.5 ng/mL.

Characterisation

Sandwich ELISA

HIV-1 p24 antibodies were tested in Colorimetric Sandwich ELISAs and sensitive matched pair combinations identified.

Standard Curve of Matched Pairs in a Native HIV-1 p24 Sandwich ELISA

 

Why Choose BBI’s HIV p24 antibodies?

+ Be confident in your results when you choose BBI’s p24 antibodies for your next assay

    - Developed and screened for performance and available exclusively from BBI Solutions

    - Multiple matched pairs with distinct binding profiles

    - Performance demonstrated in ELISA and lateral flow

    - Complementary reagents available - recombinant antigen and patient samples

+ cGMP production and quality management for increased batch-to-batch consistency

+ Customised production and conditions and gold conjugations are available on request

rHIV-1 p24 SDS-PAGE

In addition to the development of these four new antibodies, BBI Solutions is making the recombinant p24 immunogen available for purchase.  With a combined offering of high performance antibodies, recombinant capsid protein, disease state plasma, and HIV patient samples, BBI Solutions is ready to partner in the research and development of next generation HIV diagnostics.

Please enquire for samples of the antibodies or recombinant antigen.

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