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Recombinant Trypsin

CellAdvance® recombinant trypsin is a high purity recombinant protease used to detach adherent cells from a culture surface. A key enzyme used in the biopharmaceutical industry, our recombinant trypsin is expressed in Pichia pastoris and manufactured to strict GMP standards to yield a stable, high quality, contaminant-free product.

CellAdvance recombinant trypsin has a shelf life of two years from the date of manufacture. With >98% purity, this cell dissociation enzyme is suitable for a variety of uses, including stem cell culture, gene therapy, regenerative medicine, vaccine manufacture, proteomics and more.

  • Available as a lyophilised powder, or in liquid form.
  • Guaranteed animal origin-free (AOF) to eliminate risk of infectious or toxic agents.
  • Suitable for GMP cell culture; manufactured in compliance with ISO 9001:2008 standards.

Performance

Table 1: Enzyme activity using US Pharmacopeia BAEE method

Trypsin activity Rec Trypsin liquid Rec Trypsin powder Competitor
USP units/mg 4913 ± 253 4431 ± 135 3455 ± 178

 

Figure 1: Enzyme activity using US Pharmacopeia BAEE method

Recombinant Trypsin-Enzyme Activity-Graph

Cell Culturing: Dissociation characteristics of recombinant trypsin (Powder) reconstituted in 1 X PBS w/o Ca2+ + 1 mM EDTA on MRC-5, Vero, and MDCK cells:

Cell line Cell culture media Working Concentration Dissociation time (minutes)
MRC-5 MEM 0.05% w/grams of powder* 3.21 ± 0.04
Vero VP-SFM 5.40 ± 0.12
MDCK DMEM 6.14 ± 0.05

 

Table 1: Dissociation times in T25 cm2 flasks. Data is from triplicates. Cells were also visually inspected under a 4 X microscope after overnight incubation to check for morphological changes.

* Note that optimal working concentrations depend on cell lines, buffer used for preparing working solution and other experimental conditions. 1 gram of powder contains ≥ 0.6 grams of protein

Figure 2: Cell Culturing: Dissociation characteristics of recombinant trypsin reconstituted in 10 mM HCl, on MDCK cells with 5 minute incubation time and HeLa cells with 3 minute incubation time:

MDCK Cells

 

HeLa Cells

 

Ordering codes

CT-TRYP-GMP GMP Grade, Recombinant trypsin, Liquid format
CT-PTRYP-GMP GMP Grade, Recombinant trypsin, Lyophilised format

 

Downloads

Recombinant Trypsin Datasheet

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