CellAdvance® recombinant trypsin is a high purity recombinant protease used to detach adherent cells from a culture surface. A key enzyme used in the biopharmaceutical industry, our recombinant trypsin is expressed in Pichia pastoris and manufactured to strict GMP standards to yield a stable, high quality, contaminant-free product.
CellAdvance recombinant trypsin has a shelf life of two years from the date of manufacture. With >98% purity, this cell dissociation enzyme is suitable for a variety of uses, including stem cell culture, gene therapy, regenerative medicine, vaccine manufacture, proteomics and more.
- Available as a lyophilised powder, or in liquid form.
- Guaranteed animal origin-free (AOF) to eliminate risk of infectious or toxic agents.
- Suitable for GMP cell culture; manufactured in compliance with ISO 9001:2008 standards.
Table 1: Enzyme activity using US Pharmacopeia BAEE method
|Trypsin activity||Rec Trypsin liquid||Rec Trypsin powder||Competitor|
|USP units/mg||4913 ± 253||4431 ± 135||3455 ± 178|
Figure 1: Enzyme activity using US Pharmacopeia BAEE method
Cell Culturing: Dissociation characteristics of recombinant trypsin (Powder) reconstituted in 1 X PBS w/o Ca2+ + 1 mM EDTA on MRC-5, Vero, and MDCK cells:
|Cell line||Cell culture media||Working Concentration||Dissociation time (minutes)|
|MRC-5||MEM||0.05% w/grams of powder*||3.21 ± 0.04|
|Vero||VP-SFM||5.40 ± 0.12|
|MDCK||DMEM||6.14 ± 0.05|
Table 1: Dissociation times in T25 cm2 flasks. Data is from triplicates. Cells were also visually inspected under a 4 X microscope after overnight incubation to check for morphological changes.
* Note that optimal working concentrations depend on cell lines, buffer used for preparing working solution and other experimental conditions. 1 gram of powder contains ≥ 0.6 grams of protein
Figure 2: Cell Culturing: Dissociation characteristics of recombinant trypsin reconstituted in 10 mM HCl, on MDCK cells with 5 minute incubation time and HeLa cells with 3 minute incubation time:
|CT-TRYP-GMP||GMP Grade, Recombinant trypsin, Liquid format|
|CT-PTRYP-GMP||GMP Grade, Recombinant trypsin, Lyophilised format|