Why choose BBI?
+ Over 98% purity
+ Batch-to-batch consistency
+ Research-grade prices
Mammalian cell culture in a serum free medium requires an iron transporter such as transferrin. Human transferrin is a 77 kDa beta-glycoprotein with a range of phenotypes based upon the number of sialic acid residues. Each molecule of transferrin specifically binds two Fe3+ molecules.
The iron content can be adjusted to give near 100% saturation to yield holo-transferrin (Iron 1200-1600 ug/gm) or depleted to give near zero iron bound to yield apo-transferrin (Iron <50ug/gm protein) or the intermediate- sidero-transferrin (Iron 300-1000ug/gm protein).
The secondary role for transferrin in culture media is to bind endogenous metal iron which may cause cell toxicity.
|Applications||Mammalian Cell Culture|
|Concentration||300 - 600 ppm|
|Delivery Time||1 week|
|Disease Area||Plasma Proteins|
|Health and Safety||
Derived from human plasma with full traceability to the donor. Units and pools have been screened and found negative for the recommended list of infectious agents.
|Keywords||transferrin, sidero transferrin, intermediate iron transferrin|
|Presentation Matrix||Single homogenous batch, heat treated at 62°C ± 2°C for 10 hours and lyophilised from approximately 0.020M NH4HCO3 solution. May contain traces of buffer salts.|
|Product Category||Cell Culture Reagents|
|Purity/Grade||> 98% pure|
Research and manufacture
|Specification||300 - 600ppm iron content|
|Storage||Store at 2 -8°C|
|Tested Applications||cell proliferation of K562, HUT-78, RAJI and JARKUT cells.|