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Molecular

Marketing leading Enzymes from BBI Solutions  

With a strong history of innovation and quality, BBI Solutions specialises in the extraction and purification of enzymes and related biochemicals from microbial, animal and plant tissues. We have been supplying the medical diagnostics, healthcare, and pharmaceutical industries for over 65 years.  

In this time, we have developed effective, reliable and high-quality products that are used by industry leaders across the world. With international multi-site manufacturing facilities and worldwide distribution capabilities supplying a truly global and diverse customer base, BBI Solutions is the partner of choice for your reagents. 

BBI Solutions manufacture a diverse range of consistent high-quality enzymes derived from microbial, animal and plant tissues. Our enzymes, including our market-leading glucose oxidase, alkaline phosphatase and horseradish peroxidase, are renowned for their leading levels of activity and purity with a proven performance in a variety of critical diagnostic applications.  

65 years of enzyme expertise 

Our enzymes have been used by the diagnostics industry for over 65 years, within a wide range of applications, including: 

  • Biosensors 
  • Immunoassays 
  • Clinical chemistry 
  • Pharmaceutical 
  • Molecular 
  • Research 

Core Competencies  

Large product range 

Our product range includes enzymes for biosensor, immunoassay, clinical chemistry, molecular and pharmaceutical applications with a range of different grades to suit all applications.  

Quality  

We aim to develop, manufacture and supply high quality enzymes which meet both customer and regulatory requirements.  

Stability

We use quality raw materials, innovative production methods and product formulations to achieve maximum stability for our products.  We test our products regularly to ensure this remains consistent.  

Custom manufacture  

If one of our off-the-shelf products does not meet your requirements, we can offer custom development and manufacturing services to deliver an enzyme to your specification, perfect for your application. 

BBI’s capabilities 

  • Specialists in sourcing and extraction of animal and plant products – we have extensive experience in sourcing raw materials worldwide  
  • Salt/solvent fractionation – batch sizes of up to 5,000 litres 
  • Chromatography – ion exchange chromatography, affinity chromatography, size exclusion chromatography, hydrophobic interaction chromatography  
  • Crystallisation – highly effective protein purification using solvent and aqueous media  
  • Ultrafiltration – volumes of 2,500 - 30,000 litres  
  • Freeze drying – capability of 49 - 420 litres per cycle  
  • Quality control – in-process analysis and monitoring, development of assay/test procedures  
  • Product handling/sampling – we offer special packing according to customer specifications 

Please contact our Customer Services Team directly to disuss your requirements on tel:+44 (0) 1495 363000 or alternatively you can submit an enquiry here.

Enzymes FAQs

Our team are here to help! If you can’t find the answer you are looking for please contact us.

 

The material is manufactured in our ISO 13485:2016 compliant facility in Crumlin, South Wales, UK. Horseradish peroxidase and Ribonuclease are made at the ISO13485:2016 complaint facilities in Crumlin, South Wales, UK and Cape Town, South Africa

Some products can have their best before date extended beyond that stated on the ADS which arrives with the product. Contact technicalsupport@bbisolutions.com to check if the date can be extended.

• Biosensors
• Immunoassays
• Clinical chemistry
• Molecular diagnostics

BBI Solutions’ Alkaline Phosphatase (ALP) is extracted from bovine intestine then purified using multiple precipitation, fractionation, chromatography and filtration steps.

ALP is commonly used as a label in immunoassays such as ELISA, and in blotting and histochemistry. Once conjugated to antibodies, antigens, or streptavidin, its low backgrounds and linear reaction rate enables increased sensitivity over extended incubation times. It can be used with a variety of substrates producing precipitated or soluble chromogens, or with chemiluminescent and fluorogenic substrates for enhanced sensitivity.

Our high activity conjugation grade products are supplied as glycerol solutions.

We recommend you store the material between 2 - 8°C. This product is shipped at ambient temperature.

BBI grade ALPI12G, which has the highest specific activity currently available.

• Guaranteed quality: ALP is manufactured under our Quality System compliant with ISO 13485:2015, with stringent QC analysis for every batch.
• Highest activity ALP on the market: >2000 glycine U/mg protein and >6000 DEA
U/mg protein, for some batches we achieve >2300 and >7000.
• High stability: Less than 10% activity lost over 48 months.• Proven performance: We have benchmark data demonstrating proven performance against the competition.
• Multiple grades: We offer various ALP products with defined activities, enabling you to select an enzyme which best suits your application.
• Bulk extraction and purification capability: Our optimised large-scale manufacturing processes produce high volumes of all grades.

We typically pack to order and can supply any quantity from 10ku to >10,000ku.

BBI’s Glucose Oxidase is produced from large-scale fermentation of the fungus Aspergillus niger. The crude fermentation liquor is purified using multiple precipitation, fractionation, chromatography, and filtration steps.

Glucose Oxidase is used in liquid and powder glucose laboratory reagents, urine test strips, colorimetric blood glucose strips and for blood glucose monitoring in biosensors.

Our final products are lyophilised powders.

We recommend you store the material desiccated at -15°C or below. This product is shipped at ambient temperature.

• Guaranteed quality: Glucose Oxidase is manufactured under our Quality System compliant with ISO 13485:2015, with stringent QC analysis for every batch.
• Greater accuracy: Highest levels of activity and purity to increase speed and accuracy
• Choice and flexibility: Multiple grades and custom preparations available for specific applications to optimise your assay at reduced cost
• Proven performance: Enabling strip manufacturers to comply with the latest accuracy guidelines (+/- 15%)
• Batch to batch reproducibility and consistency: Optimised and controlled manufacturing procedures to ensure reproducibility and consistency – essential in high volume strips
• High manufacturing capacity: BBI has bulk manufacturing capabilities

We typically pack to order and can supply any quantity from 1MU to >1,000MU.

BBI’s Peroxidase is extracted from horseradish roots then purified using multiple
precipitation, fractionation, chromatography and filtration steps.

Horseradish Peroxidase (HRP) is used in a wide range of liquid clinical chemistry reagents, dry powder reagents and test strips It is also commonly used as a label in immunoassays such as ELISA, and in blotting and histochemistry. Once conjugated to antibodies, antigens, or streptavidin, its rapid turnover rate gives high sensitivity over short incubation times.

Our final products are lyophilised powders.

We recommend you store the material desiccated at -15°C or below. This product is shipped at ambient temperature.

• Guaranteed quality: HRP is manufactured under our Quality System compliant with ISO 13485:2015, with stringent QC analysis for every batch.
• Controlled and consistent supply: We have developed close relationships with horseradish growers to ensure planned purchase of specially selected and cultivated horseradish roots
• High isozyme C content: Material available above 90%
• High activity levels: ~280 U/mg
• Highly available amino groups: For clean conjugation
• Bulk extraction capability: Our optimised large-scale manufacturing processes produce high volumes of both clinical chemistry and immunoassay grades

We typically pack to order and can supply any quantity from mg to kg depending on the grade required.

BBI’s Ribonuclease is extracted from bovine pancreas using multiple precipitation, fractionation, chromatography and filtration steps.

Ribonuclease can be used in the following applications:
• In isolation of DNA for RNA-free DNA, especially plasmid DNA• Structural studies of RNA
• The depolymerisation of viscous biological suspensions, rendering RNA-free solutions

Our final products are lyophilised powders.

We recommend you store the material desiccated at -15°C. This product is shipped at ambient temperature.

• Guaranteed quality: RNase is manufactured under our Quality System compliant with ISO 13485:2015, with stringent QC analysis for every batch.
• Proven performance: In DNA extraction kits
• Batch-to-batch reproducibility: Enabling consistent formulations
• Bulk extraction and purification capability: Our optimised large-scale manufacturing processes produce high volumes of purified RNase
• Secure supply: We have direct relationships with our raw material suppliers to ensure a secure supply chain

We typically pack to order and can supply any quantity from g to kg.

BBI’s urease is obtained from specially selected and cultivated jack beans. The enzyme is extracted from the beans then purified using multiple precipitation, fractionation, chromatography and filtration steps.

Urease is used in liquid and powder urea laboratory reagents, and biosensors. It can be used in the determination of urea in biological fluids (couple with GLDH, in the ultraviolet method). Urease can also be used as an enzymes label in ELISA.

Our final products are lyophilised powders.

We recommend you store the material desiccated at -15°C or below. This product is shipped at ambient temperature.

• Guaranteed quality: Urease is manufactured under our Quality System compliant with ISO 13485:2015, with stringent QC analysis for every batch.• Proven performance: In liquid and powder reagents
• High purity: For biosensor applications
• Bulk capability: Manufacturing in high volumes to reduce cost
• Batch-to-batch reproducibility: Enabling consistent formulations
• Secure supply: We have direct relationships with our raw material suppliers to
ensure a secure supply chain

We typically pack to order and can supply any quantity from 1MU to >1,000MU

Enzymes for Molecular Biology 

BBI Solutions  is the leading manufacturer of Ribonuclease A, supplying the enzyme as a key component for molecular biology reagents. The enzyme has been used for the removal of RNA in DNA purification procedures for over 25 years. Our raw material selection and production procedures have been optimised to ensure that we achieve the highest quality, stability and batch-to-batch consistency, and provide RNase products with proven performance in DNA extraction kits. 

Ribunuclease

Ribonuclease (RNase) is classified as an endonuclease, which specifically cleaves phosphodiester bonds at the 3’-end of pyrimidine nucleosides and at the 5’-ribose of a nucleotide, ribonucleic acid (RNA). Ribonuclease can be used in the following applications: 

  • In isolation of DNA for RNA-free DNA, especially plasmid DNA 
  • Structural studies of RNA 
  • The depolymerisation of viscous biological suspensions, rendering RNA-free solutions 

 

Technical Documents

If you require any further product specific technical information or datasheets please submit your request to our technical team here 

Last updated: 26/03/2021

BBI Solutions Products and Services Brochure

Last updated: 26/03/2021

BBI Solutions Enzymes Brochure

Last updated: 01/02/2021

Ribunoclease

Last updated: 26/03/2021

BBI Solutions Products and Services Brochure

Last updated: 26/03/2021

BBI Solutions Enzymes Brochure

There are currently no documents in this category.
Last updated: 01/02/2021

Ribunoclease

There are currently no documents in this category.
There are currently no documents in this category.
There are currently no documents in this category.
There are currently no documents in this category.
There are currently no documents in this category.
There are currently no documents in this category.

Request a callback

If you have any questions or wish to discuss your requirements please submit an enquiry using the form below. Our dedicated customer services team will respond directly.

BBI Solutions Brands

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The acquisition of DIARECT in June 2020 and their world leading recombinant autoimmune antigen capability immediately enhances BBI’s portfolio and position as a ‘complete’ immunoassay reagent supplier. This further enhances BBI’s position as the world’s largest diagnostics components company with a market leading antigen portfolio.

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Maine Biotechnology Services brings 27 years of knowledge, experience, process development, and project management to our customers to ensure the best possible antibodies for any application. As antibody development goals in the biotechnology and pharmaceutical industry have evolved the acquisition of MBS will help BBI further meet and exceed our customers needs.

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