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Substrates

Surmodics Substrates for proven performance 

BBI Solutions offer specific and sensitive IVD signal solutions with proven performance 

The right substrate can make a substantial contribution to the overall sensitivity of your assay. The following BioFX® substrates with different kinetic formulations give you the freedom to choose the substrate that optimizes the dynamic range of your immunoassay without compromising assay sensitivity.

Please contact our Customer Services Team directly to discuss your requirements on tel:+44 (0) 1495 363000 or alternatively you can submit an enquiry here.

TMB AND ABTS HRP MICROWELL SUBSTRATES

Product Product code Quantity Description
TMB High Definition One
Component HRP Microwell
Substrate
TMBHD-0100-01
TMBHD-1000-01
100 mL
1000 mL
The latest member of the BioFX™ TMB Substrate family with
the fastest kinetic properties in thisrange as well as outstanding sensitivity.
TMB Super Sensitive One
Component HRP
Microwell Substrate
TMBS-0100-01
TMBS-1000-01
100 mL
1000 mL
•  Our most sensitive one-component TMB formulation
•  Up to 40% more sensitive than TMBW
TMB One Component HRP
Microwell Substrate
TMBW-0100-01
TMBW-1000-01
100 mL
1000 mL
•  Our most popular formulation
•  Suitable for use in virtually every quantitative or qualitative test
TMB Conductivity One
Component HRP
Microwell Substrate
TMBC-0100-01
TMBC-1000-01
100 mL
1000 mL
•  As robust as TMBW
•  Formulated specifically for liquid level detection systems on instruments
•  Up to 10% more sensitive than TMBW
TMB Slow Kinetic One
Component HRP
Microwell Substrate
TMSK-0100-01
TMSK-1000-01
100 mL
1000 mL
•  A slower kinetic formulation to allow a wider dynamic/working range without compromising  assay performance
•  Up to 25% less sensitive than TMBW
TMB Super Slow One
Component HRP Microwell
Substrate
TTMB-0100-01
TTMB-1000-01
100 mL
1000 mL
•  Up to 35% less sensitive than TMBW  without compromising assay performance
TMB Double Slow One
Component HRP Microwell
Substrate
TMDS-0100-01
TMDS-1000-01
100 mL
1000 mL
•  A doubly slow kinetic option for consistent results
•  Up to 60% less sensitive than TMBW

TMB Extended Range
HRP Microwell Substrate

TMBX-0100-01
TMBX-1000-01

100 mL
1000 mL
•  Our slowest kinetic option for extended dynamic range and improved reproducibility
ABTS One Component HRP
Microwell Substrate -
Extended Shelf Life
ABTS-0100-01
ABTS-1000-01
100 mL
1000 mL
•  Three year stability while offering the sensitivity needed for your ELISA assay
•  Much more stable background compared to standard ABTS formulations

 

TMB HRP MEMBRANE SUBSTRATES

Product Product code Quantity Description
TMB Enhanced One Component
HRP Membrane Substrate
ESPM-0100-01
ESPM-1000-01
100 mL
1000 mL
•  Room temperature stability for 2 years, stable for 4 years at 4°C
•  Similar sensitivity to most mid to high level chemiluminescent membrane substrates
•  Extended dynamic range with reduction of wash-out at higher peroxidase concentrations
TMB One Component
HRP Membrane
Substrate
TMBM-0100-01
TMBM-1000-01
100 mL
1000 mL
•  A high quality and economical precipitating TMB option for Western blotting and other membrane applications

 

AP MICROWELL SUBSTRATES

Product Product code Quantity Description

AP-Yellow One Component Microwell Substrate
(pNPP) with Stabilizing Pellets

PNPS-0100-01
PNPS-1000-01
100 mL
1000 mL
•  No spontaneous colour change
•  Long shelf life

 

AP MEMBRANE SUBSTRATES

Product Product code Quantity Description
BCIP/NBT Blue One
Component AP Membrane Substrate
BCIB-0100-01
BCIB-1000-01
100 mL
1000 mL
•  Blue precipitate
•  No unpredictable colour change
•  Improved sensitivity with low background
BCIP/NBT Purple One
Component AP Membrane Substrate
BCID-0100-01
BCID-1000-01
100 mL
1000 mL
•  Purple precipitate
•  No unpredictable colour change
•  Improved sensitivity with low background

 

Related Products

If you require any further product specific technical information or datasheets please submit your request to our technical team here 

BioFX™ brand range of substrate solutions  

BBI offers the  BioFX™ brand range of substrate solutions, designed primarily for use in IVD applications, to our EU clients. The range has ready to use substrate solutions for both Horse Radish Peroxidase and Alkaline Phosphatase enzyme, complementing the BBI manufactured enzyme products. 

The BioFX™ TMB family has a broad range of kinetic rates with low background and improved signal‐to‐noise ratios that improve assay performance.  The BioFX® TMB One Component HRP Microwell Substrate contains 3,3’,5,5’‐tetramethylbenzidine (TMB) and is supplied as a ready‐to‐use solution.  Peroxidase reacts with the substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620‐650 nm for ELISA applications.  Stop solutions can be added to prevent further colour development and reading the absorbance values at the desired wavelengths.  

BioFX™ alkaline phosphatase substrates contain p‐nitrophenylphosphate (p‐NPP) and are supplied as formulated solutions for immunoassay applications.  A soluble yellow product is obtained when this substrate is reacted with alkaline phosphatase. 

 

Technical Documents

If you require any further product specific technical information or datasheets please submit your request to our technical team here 

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