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Substrates

BBI Substrates for proven performance 

BBI Solutions offer specific and sensitive IVD signal solutions with proven performance 

The right substrate can make a substantial contribution to the overall sensitivity of your assay. The following BioFX® substrates with different kinetic formulations give you the freedom to choose the substrate that optimizes the dynamic range of your immunoassay without compromising assay sensitivity.

Please contact our Customer Services Team directly to discuss your requirements on tel:+44 (0) 1495 363000 or alternatively you can submit an enquiry here.

Related Products

If you require any further product specific technical information or datasheets please submit your request to our technical team here 

ABTS One Component HRP Microwell Substrate - Extended Shelf Life

ABTS

ABTS One Component HRP Microwell Substrate – Extended Shelf Life contains 2.2’-azino-di[3-ethylbenzthiazoline sulfonate (6)] in a mild acidic buffer
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BCIP/NBT Blue One Component AP Membrane Substrate

BCIB-1000-01

BCIP/NBT Blue One Component AP Membrane Substrate contains 5-bromo-4-chloro-3-indolyl-phosphate and nitroblue tetrazolium. The substrate is a ready to use solution
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BCIP/NBT Purple One Component AP Membrane Substrate

BCID-1000-01

BCIP/NBT Purple One Component AP Membrane Substrate contains 5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium. The substrate is a ready to use solution
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TMB Enhanced One Component HRP Membrane Substrate

ESPM-0100-01

TMB Enhanced One Component HRP Membrane Substrate is a ready to use solution containing 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. This enhanced formulation extends the dynamic rang...
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TMB Enhanced One Component HRP Membrane Substrate

ESPM-1000-01

BioFX™ TMB Enhanced One Component HRP Membrane Substrate is a ready‐to‐use solution containing 3,3’,5,5’‐ tetramethylbenzidine in a mildly acidic buffer
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AP-Yellow One Component Microwell Substrate (pNPP) with Stabilizing Pellets

PNPS

BioFX™ AP‐Yellow One Component Microwell Substrate with Stabilizing Pellets contains p‐nitrophenylphosphate (p‐NPP) and is supplied as a one component solution for immunoassay applications
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TMB Conductivity One Component HRP Microwell Substrate

TMBC

TMB Conductivity One Component HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution
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TMB One Component HRP Membrane Substrate

TMBM

TMB One Component HRP Membrane Substrate is a ready to use solution containing 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer
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TMB Super Sensitive One Component HRP Microwell Substrate

TMBS

TMB Super Sensitive One Component HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution
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TMB One Component HRP Microwell Substrate

TMBW

TMB One Component HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution
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TMB Extended Range HRP Microwell Substrate

TMBX

TMB Extended Range HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution
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TMB Double Slow One Component HRP Microwell Substrate

TMDS

TMB Double Slow One Component HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution
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TMB Slow Kinetic One Component HRP Microwell Substrate

TMSK

TMB Slow Kinetic One Component HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution
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TMB Super Slow One Component HRP Microwell Substrate

TTMB

TMB Super Slow One Component HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution
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BioFX™ brand range of substrate solutions  

BBI offers the  BioFX™ brand range of substrate solutions, designed primarily for use in IVD applications, to our EU clients. The range has ready to use substrate solutions for both Horse Radish Peroxidase and Alkaline Phosphatase enzyme, complimenting the BBI manufactured enzyme products. 

The BioFX™ TMB family has a broad range of kinetic rates with low background and improved signal‐to‐noise ratios that improve assay performance.  The BioFX® TMB One Component HRP Microwell Substrate contains 3,3’,5,5’‐tetramethylbenzidine (TMB) and is supplied as a ready‐to‐use solution.  Peroxidase reacts with the substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620‐650 nm for ELISA applications.  Stop solutions can be added to prevent further colour development and reading the absorbance values at the desired wavelengths.  

BioFX™ alkaline phosphatase substrates contain p‐nitrophenylphosphate (p‐NPP) and are supplied as formulated solutions for immunoassay applications.  A soluble yellow product is obtained when this substrate is reacted with alkaline phosphatase. 

 

Technical Documents

If you require any further product specific technical information or datasheets please submit your request to our technical team here 

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DIARECT Immunodiagnostic Brochure

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