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ELISA Protein Stabilisers/Blockers/Diluents

Surmodics Stabilisers to improve assay performance

BBI offers a range of protein and non-protein based reagents designed to improve the performance of IVD assays. BBI supplies the Surmodics branded range of stabilisers to it EU based clients. StabilCoat™, StabilBlock™ and StabilGuard™ product ranges effectively preserve the conformation and activity of proteins while simultaneously blocking the surface to reduce non‐specific binding. The products can be used to stabilise antibodies, antigens or enzymes on an assortment of immunoassay components: polystyrene plates, tubes, glass, membranes, and filter paper.  

The non‐specific binding of serum proteins, conjugates and other assay components to solid surfaces in immunoassay applications requires that a blocking step be incorporated into the protocol to maximize assay sensitivity.  Additionally, the manufacturing of immunoassay test kits requires long-term protein stability to increase the manufacturing scale and extend shelf lives for the end user.  The different formulations allow these requirements to be accomplished in a single step while easily being substituted for the blocking solution in an existing assay protocol. 

StabilZyme™ conjugate/protein stabilisers protect the entire conjugate by preventing the loss of catalytic activity and maintaining the structural integrity of the protein in solution. These stabilisers allow for the storage of conjugated proteins at lower use concentrations, extended shelf life and increased signal‐to‐noise ratios for improved assay performance. Materials are provided as ready to use formulations or concentrates in specific buffered matrix designed for optimal performance.  

Please contact our Customer Services Team directly to discuss your requirements on tel:+44 (0) 1495 363000 or alternatively you can submit an enquiry here.

 

IMMUNOASSAY STABILIZERS / BLOCKERS

Product Product code Quantity Description
StabilCoat® Immunoassay Stabilizer SC01-0050
SC01-1000
50 mL
1000 mL
Effectively preserves the conformation and activity of dried proteins.
5X StabilCoat® Immunoassay Stabilizer SC05-0100
SC05-1000
100 mL
1000 mL
• 5x concentrate
• Effectively preserves the conformation and activity of dried proteins
StabilGuard® Immunoassay Stabilizer (BSA-Free) SG01-0050
SG01-1000
50 mL
1000 mL
Maintains the activity of antibodies and other biomolecules absorbed or immobilised to solid substrates.
StabilBlock® Immunoassay Stabilizer

ST01-0050
ST01-1000

50 mL
1000 mL
Minimizes non-specific binding to lower detection limits and maximizes signal-to-noise ratios of immunoassays.
StabilBlock® Immunoassay Stabilizer-CMIT/MIT ST01-CF01-0050
ST01-CF01-1000
50 mL
1000 mL
Newly developed formulation containing a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative.

 

STABILIZERS FOR MICROSPHERE-BASED ASSAYS AND MICROARRAY SLIDES

Product Product code Quantity Description
StabilCoat® Plus Microarray Stabilizer SC02-0050
SC02-1000
50 mL
1000 mL
Improves the stability of proteins bound to microspheres stored in solution, and array proteins stored in the dried state.
StabilGuard® Choice Microarray Stabilizer (BSA-Free) SG02-0050
SG02-1000
50 mL
1000 mL
Improves the stability of proteins bound to microspheres stored in solution, and array proteins stored in the dried state.

 

PROTEIN AND CONJUGATE STABILIZERS

Product Product code Quantity Description
StabilZyme® AP Conjugate Stabilizer SA01-0050
SA01-1000
50 mL
1000 mL
Developed specifically for Alkaline Phosphatase (AP) conjugates in solution. It prevents the loss of catalytic activity and maintains stability of proteins in solution.
StabilZyme® AP Conjugate Stabilizer-CMIT/MIT

SA01-CF01-0050
SA01-CF01-1000
SA01-CF01-2000

50 mL
1000 mL
2000 mL
This alternative formulation contains a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative.
StabilZyme® Protein-Free AP Stabilizer SAPF-0050
SAPF-1000
50 mL
1000 mL
Optimised to prevent the loss of Alkaline Phosphatase (AP) activity while maintaining the conformational structure and binding efficacy of the conjugated antibody.
StabilZyme® Protein-Free AP Stabilizer-CMIT/MIT

SAPF-CF01-0050
SAPF-CF01-1000

50 mL
1000 mL
This alternative formulation contains a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative.
StabilZyme® HRP Conjugate Stabilizer

SZ02-0050
SZ02-1000

50 mL
1000 mL
Developed specifically for Horseradish Peroxidase (HRP) conjugates in solution and is readily incorporated into virtually any assay production protocol.
StabilZyme® HRP Conjugate Stabilizer-CMIT/MIT

SZ02-CF02-0050
SZ02-CF02-1000

50 mL
1000 mL
This alternative formulation contains a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative.
StabilZyme SELECT® Stabilizer

SZ03-0050
SZ03-1000

50 mL
1000 mL
Stabilises biologically active components in solution and protects the activity of proteins commonly used in immunoassays, such as antisera controls and HRP conjugates.
StabilZyme SELECT® Stabilizer-CMIT/MIT

SZ03-CF02-0050
SZ03-CF02-1000

50 mL
1000 mL
This alternative formulation contains a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative.
StabilZyme® NOBLE Stabilizer (BSA-Free)

SZ04-0050
SZ04-1000

50 mL
1000 mL
Recommended for biologically active components in solution, including enzyme conjugates, conjugated proteins, antibody-coated particles, and unmodified proteins.
StabilZyme® NOBLE Stabilizer (BSA-Free) CMIT/MIT SZ04-CF02-0050
SZ04-CF02-1000
50 mL
1000 mL
This alternative formulation contains a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative.
StabilZyme® Protein-Free Stabilizer

SZPF-0050
SZPF-1000

50 mL
1000 mL
Designed for conjugated proteins, antibody-coated particles, and unmodified proteins in solution. It delivers top performance in a protein-free formulation.
StabilZyme® Protein-Free
Stabilizer-CMIT/MIT

SZPF-CF01-0050
SZPF-CF01-1000

50 mL
1000 mL
This alternative formulation contains a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative.

 

STABILBLOT™ BLOCKERS

Product Product code Quantity Description
StabilBlot™  Protein-Free Blocker SB01-0125-01
SB01-1000-01
125 mL
1000 mL
•  Ready to use
•  The absence of protein in the formulation provides a synthetic alternative that eliminates cross-reactivity and increases sensitivity
StabilBlot™ Milk Blocker (10X) SB04-0125-01
SB04-1000-01
125 mL
1000 mL
•  10x concentrate
•  For the strongest blocking, the milk formulation is recommended
StabilBlot™ Casein Blocker (5X, TBS) SB05-0125-01
SB05-1000-01
125 mL
1000 mL
•  5x concentrate
•  For strong blocking of background, a casein TBS and a casein PBS formulation are available

 

ASSAY DILUENTS

Product Product code Quantity Description
Surmodics Assay Diluent (Protein-Free) SM01-0050
SM01-1000
50 mL
1000 mL
Surmodics Assay Diluent reduces matrix interference in samples including heterophilic antibodies such as HAMA (human anti-mouse antibody) and RF (rheumatoid factor).
Surmodics Assay Diluent (Protein-Free)-CMIT/MIT SM01-CF02-0050
SM01-CF02-1000
50 mL
1000 mL
New formulation of Surmodics Assay contains a mixture of 5-Chloro-2-Methyl-3(2H)-isothiazolone and 2-Methyl-3(2H)-isothiazolone as preservative.
Surmodics MatrixGuard™ Diluent SM02-0050
SM02-1000
50 mL
1000 mL
Provides unsurpassed blocking whereby matrix interferences are effectively blocked while the intended assay signal is maintained.

 

Related Products

If you require any further product specific technical information or datasheets please submit your request to our technical team here 

Stabiliser properties

BBI offers the Surmodics brand range of IVD assay stabilisers.  Surmodics’ immunoassay stabilisation products preserve the conformation of proteins in their native folded state when dried or lyophilized and then coated to a range of surfaces, including microtiter plates, latex beads and magnetic particles. This stability gives you confidence in the viability of the antigens and/or antibodies you incorporate as key components of your diagnostic tests.  

The non‐specific binding of serum proteins, conjugates and other assay components to solid surfaces in immunoassay applications requires that a blocking step be incorporated into the protocol to maximise assay sensitivity.  Additionally, the manufacturing of immunoassay test kits requires long-term protein stability to increase the manufacturing scale and extend shelf lives for the end user.  The StabilCoat™, StabilGuard™ and StabilBlock™ formulations are adapted to specific applications and allow for these requirements to be accomplished in a single step while easily being substituted for the blocking solution in an existing assay protocol. These materials have been designed in buffered solutions that prevent non-specific molecules binding to active surfaces, decreasing background signal and enhancing long-term stability of biological products. The specific product most suited to the application can be determined by experiment.  

Generating protein arrays requires assurance that the biologically active structure of proteins remains intact and stable in the presence of external factors, such as temperature, pH, metal ions, and other contaminants. Surmodics’ stabilisation products for protein microarrays preserve the conformation of proteins in their native, folded state when coated to surfaces such as microarray slides or beads used in multiplexing protein assays. The StabilZyme™ range of conjugate stabilisers is optimised to maintain the conformation of antibodies/antigens conjugated to signal enzymes such as BBI’s Alkaline Phosphatase in solution.  Adding the conjugate directly into the  solution allows for stable storage of the conjugate at a working concentration and eliminates the need for subsequent dilutions.    

Technical Documents

If you require any further product specific technical information or datasheets please submit your request to our technical team here 

Last updated: 19/10/2020

DIARECT product catcalogue

Last updated: 19/10/2020

DIARECT product catcalogue

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