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Autoimmune and Inflammation

Autoimmune and inflammation biomarkers from BBI

BBI offers an extensive range of inflammation biomarker and autoimmune products derived either from native human tissue sources or using carefully chosen recombinant expression systems. All source materials are screened for safety using the latest sensitive methods. Products are made to order or available from stock using processes defined by specific batch records. 

We endeavour to always supply material to our customers’ timelines but rely on our clients to provide forecasts and estimates of usage.  Materials are prepared using a range of protein expression strategies and purification technologies, some unique to BBI.

Products are mainly available as highly purified preparations,  are formulated to optimise shelf-life and provide material in the most convenient storage format. Products are often tested with or referenced against specific stored patient samples to ensure the performance.

Please contact our Customer Services Team directly to disuss your requirements on tel:+44 (0) 1495 363000 or alternatively you can submit an enquiry here.

Disease Area	Product code	Product	Quantity
Systemic Autoimmue Rheumatic Diseases	30300 30301	DFS70	0.1 mg 1.0 mg
Mixed Connective Tissue Disease / SLE	13000 13001	U1-snRNP 68/70 kDa	0.1 mg 1.0 mg
	13100 13101	U1-snRNP A	0.1 mg 1.0 mg
	13200 13201	U1-snRNP C	0.1 mg 1.0 mg
	13300 13301	U-snRNP B/B‘	0.1 mg 1.0 mg
Systemic Lupus Erythematosus (SLE)	17500 17501	Sm (non recombinant; bovine)	0.1 mg 1.0 mg
	11600 11601	RNP/Sm (non recombinant; bovine)	0.1 mg 1.0 mg
	11700 11701	SmD	0.1 mg 1.0 mg
	11800 11801	SmD1	0.1 mg 1.0 mg
	11900 11901	SmD2	0.1 mg 1.0 mg
	12000 12001	SmD3	0.1 mg 1.0 mg
	31000 31001	Nucleosome (non recombinant; bovine)	0.1 mg 1.0 mg
	31100 31101	Histone (non recombinant; bovine)	0.1 mg 1.0 mg
	12300 12301	dsDNA (plasmid)	0.1 mg 1.0 mg
	14100 14101	Ribosomal Phosphoprotein P0	0.1 mg 1.0 mg
	14200 14201	Ribosomal Phosphoprotein P1	0.1 mg 1.0 mg
	14300 14301	Ribosomal Phosphoprotein P2	0.1 mg 1.0 mg
	15400 15401	Proliferating Cell Nuclear Antigen (PCNA)	0.1 mg 1.0 mg
	17300 17301	Ku (p70/p80)	0.1 mg 1.0 mg
	19700 19701	Nucleolin	0.1 mg 1.0 mg
Sjögren's Syndrome / SLE	17400 17401	Ro/SS-A (60 kDa; recombinant)	0.1 mg 1.0 mg
	15500 15501	Ro/SS-A (60 kDa; non recombinant; bovine)	0.1 mg 1.0 mg
	12700 12701	Ro/SS-A (52 kDa)	0.1 mg 1.0 mg
	12800 12801	La/SS-B	0.1 mg 1.0 mg
Systemic Sclerosis / CREST Syndrome	12500 12501	Centromere Protein B (CENP-B)	0.1 mg 1.0 mg
	16900 16901	Centromere Protein A (CENP-A)	0.1 mg 1.0 mg
	12400 12401	DNA Topoisomerase I (Scl-70; full length)	0.1 mg 1.0 mg
	14500 14501	DNA Topoisomerase I (Scl-70; truncated)	0.1 mg 1.0 mg
	11500 11501	DNA Topoisomerase I (Scl-70; non recombinant; bovine)	0.1 mg 1.0 mg
	16000 16001	PM/Scl 100	0.1 mg 1.0 mg
	17000 17001	PM/Scl 75	0.1 mg 1.0 mg
Myositis	12900 12901	Histidyl-tRNA Synthetase (Jo-1)	0.1 mg 1.0 mg
	15600 15601	Threonyl-tRNA Synthetase (PL-7)	0.1 mg 1.0 mg
	15700 15701	Alanyl-tRNA Synthetase (PL-12)	0.1 mg 1.0 mg
	11100 11101	Glycyl-tRNA Synthetase (EJ)	0.1 mg 1.0 mg
	30100 30101	Asparaginyl-tRNA Synthetase (KS)	0.1 mg 1.0 mg
	18400 18401	SRP54	0.1 mg 1.0 mg
	16000 16001	PM/Scl 100	0.1 mg 1.0 mg
	17000 17001	PM/Scl 75	0.1 mg 1.0 mg
	18100 18101	Mi-2	0.1 mg 1.0 mg
	11000 11001	TIF1 gamma	0.1 mg 1.0 mg
	31700 31701	NXP2	0.1 mg 1.0 mg
	31600 31601	SAE1/SAE2	0.1 mg 1.0 mg
	30000 30001	MDA5	0.1 mg 1.0 mg
Autoimmune Thyroid Diseases	12100 12101	Thyroid Peroxidase (TPO)	0.1 mg 1.0 mg
Autoimmune Thyroid Diseases	12200 12201	Thyroglobulin (non recombinant)	0.1 mg 1.0 mg
Celiac Disease	15200 15201	Tissue Transglutaminase (tTG; expressed in Baculovirus/Sf9)	0.1 mg 1.0 mg
	14400 14401	Tissue Transglutaminase (tTG; expressed in E. coli)	0.1 mg 1.0 mg
	19500 19501	Gliadin (recombinant; deamidated)	0.1 mg 1.0 mg
	31500 31501	Gliadin (non recombinant)	0.1 mg 1.0 mg
Autoimmune Liver Diseases	31800 31801	Cytochrome P450 2D6 (LKM 1; ng)	0.1 mg 1.0 mg
	13700 13701	Formiminotransferase Cyclodeaminase (LC 1)	0.1 mg 1.0 mg
	30800 30801	SLA/LP	0.1 mg 1.0 mg
	18000 18001	M2	0.1 mg 1.0 mg
	17700 17701	BCOADC-E2	0.1 mg 1.0 mg
	17800 17801	OGDC-E2	0.1 mg 1.0 mg
	17900 17901	PDC-E2	0.1 mg 1.0 mg
	18900 18901	Sp100	0.1 mg 1.0 mg
	19000 19001	gp210	0.1 mg 1.0 mg
	19400 19401	Nup62	0.1 mg 1.0 mg
ANCA associated Diseases	18500 18501	Myeloperoxidase (MPO; non recombinant)	0.1 mg 1.0 mg
	18600 18601	Proteinase 3 (PR3; non recombinant)	0.1 mg 1.0 mg
	19200 19201	BPI (non recombinant)	0.1 mg 1.0 mg
Antiphospholipid Syndrome / Thromboembolic Syndrome	14900 14901	ß2-Glycoprotein 1 (Apolipoprotein H)	0.1 mg 1.0 mg
	11300 11301	ß2-Glycoprotein 1 (Apo H; non recombinant; human)	0.1 mg 1.0 mg
	11400 11401	ß2-Glycoprotein 1 (Apo H; non recombinant; bovine)	0.1 mg 1.0 mg
Anti-GBM Disease	16800 16801	Glomerular Basement Membrane (GBM; dissociated)	0.1 mg 1.0 mg
Crohn's Disease	19600 19601	GP2	0.1 mg 1.0 mg
Diabetes Mellitus	31900 31901	Glutamate Decarboxylase 65 kDa (GAD65; ng)	0.1 mg 1.0 mg
Diabetes Mellitus	30500 30501	IA-2 (ICA 512)	0.1 mg 1.0 mg
Pernicious Anemia	16700 16701	Intrinsic Factor	0.1 mg 1.0 mg

Inflammation markers

Product description	Product code
Alpha-1-Antichymotrypsin	P159-5
Alpha-1-antitrypsin	P165-5
Complement C1q	P102-1
Complement C4c	P151-8
Human C-Reactive Protein (CRP)	P100-0
Human C-Reactive Protein (CRP)	P100-7N
Prealbumin	P171-1
Prealbumin	P171-2
Recombinant Human Procalcitonin (Recombinant)	P742-1

Antigens FAQs

Our team are here to help! If you can’t find the answer you are looking for contact us.

Download as PDF

For our European customers please contact your BBI sales representative or reach out to our customer services team. For our US and Asia customers you can also contact your BBI representative for support however sales enquiries, where relevant, will be routed through a local representative. In the US for DIARECT catalogue products, these should be ordered from our US distribution partner Surmodics.

Upon receipt, our antigens / antibodies should be stored frozen at - 70°C or below until use. We recommend thawing at room temperature, but thawed products should not remain at room temperature for extended periods of time. Mix products gently before use. If you plan on using only a portion of the product, please thaw and dispense it into aliquot volumes that are most practical for your application and store frozen at - 70°C or below. Please note that we cannot guarantee the shelf life after the antigens / antibodies have been re-dispensed and adviceagainst repeated freeze thaw cycles.

While general recommendations for antigen / antibody storage conditions and buffers are given in the antigen's / antibody's specification sheet, which can be found in the “+INFO” section associated with each antigen / antibody on DIARECT's website, the detailed buffer composition is available together with the lot-specific Certificate of Analysis (CoA).

No, we do not recommend using antigens / antibodies without a guaranteed cold chain. If you receive a thawed product please inform our customer service immediately. Regarding dry ice shipments please visit section "shipping information" on our website for detailed information on temperature
tests.

For each antigen / antibody, DIARECT created a “+INFO” section on its website, which contains the antigen's / antibody's material safety data sheet (MSDS) and specification sheet. The latter provides general recommendations regarding antigen / antibody storage and buffers. For select antigens / antibodies, the “+INFO” section also contains a brochure offering some background information and immunological test data. You can also contact your local representative or info-dia@bbisolutions.com for more details.

Most of our antigens have a guaranteed shelf life of five years unless otherwise noted on our Certificates of Analysis (CoA) and data sheets, and providing that they are kept frozen at -70°C or below. For the shelf life of our antibodies, please check the expiry date on the lot-specific CoA provided with the product.

We guarantee the reservation of a specified amount of antigen(s). Typically, customers reserve the amount of antigen(s) needed for a manufacturing period of up to 18 months. You simply inform us of
your antigen requirements for this period, and every 4 months you will receive an updated overview regarding the accuracy of your estimate. The reservation system also includes the allocation of early
enough samples of new lots of the respective antigen(s). This enables the customer to evaluate the new lot at an early stage for future reservations and allows a smooth transition to this new lot.
Advantages provided by DIARECT's reservation system:
- Security of supply with no additional costs
- No need for continual readjustment and fine tuning of your assay because of new antigen lots

BV and Ec represent the type of expression system that was used to produce the antigen of interest. BV stands for DIARECT's baculovirus/insect cell expression system, while Ec stands for DIARECT's E. coli expression system.

For purification, the preferred technique is the very effective, yet gentle immobilized metal-ion affinity chromatography (IMAC) in combination with His-tag technology, which yields physiologically and
immunologically active proteins. Our native proteins are purified using appropriate standard chromatography purification methods including ion exchange, size exclusion and immunoaffinity.

DIARECT's recombinant and native antigens / antibodies - always produced according to documented procedures (SOPs) and manufactured per EN ISO 13485 and 9001 standards - undergoing a rigorous series of tests in our quality control laboratory. Prior to its approval and release, each lot is subjected to various biochemical and immunological analyses to assure conformance to our strict product specifications. The goal of these extensive inspections is to ensure lot-to-lot conformance, which guarantees consistent results.

General information regarding the origin and expression constructs of antigens are provided in the respective specification sheets, however, exact sequence data are considered proprietary information. Most recombinant antigen preparations are supplied with a 6xHis-tag as part of the sequence.

DIARECT antigens are manufactured in our EN ISO 13485 and EN ISO 9001 audited facility in Freiburg, Germany. Supply is either direct from our Freiburg facility or may be managed by our local representatives in specific territories.

Why choose Autoimmune antigens from BBI Solutions?

BBI and our scientists in Freiburg are world renowned for providing a comprehensive and high-quality range of autoimmune antigens. Many of these antigens are reference materials for some rare and important disease assays.

Our autoimmune antigens are used in many primary screening methods for determination of the specific target for a serological autoimmune response. The company objective has been to derive an extensive list of targets for specific disease states where high specification and highly reproducible products are required to give the best performance across different assay platforms including ELISA, microarray and line assay.

In addition, BBI supply a comprehensive range of serum biomarker indicators of inflammation such as CRP and Procalcitonin. These materials are routinely used as critical reagents to prepare IVD assay standards and quality control materials. BBI derives its range of autoimmune antigens by molecular methods and the proteins are expressed in specific host organisms. The host cell lines are stored and propagated in our bioreactor systems. Some of our recombinant products are expressed with specific His-tag sequences to facilitate purification by nickel chelate affinity chromatography.

Some of our inflammation biomarkers are isolated as native products from human plasma or tissue sources. All human derived source material is always screened for viral safety for markers such as HIV, Hepatitis B and C prior to use. These native and untagged products are purified by a sequential series of different chromatography techniques. The purified proteins are then formulated liquid or lyophilised in the most appropriate stabilising buffer and filtered to 0.2µm to ensure low bioburden. 

Quality embedded at every step of the way

Products are characterised prior to fill to determine the recovery and purity of the protein using a range of analytical techniques. Techniques such as Coomassie stained SDS-PAGE are used to test and verify the product purity. Importantly, our autoimmune markers for serological applications are tested against a defined panel of patient samples. The immunological recovery of our inflammatory biomarkers is tested by established immunoassay methods.

After the products have been filled and formulated, they are retested by our independent Quality Control laboratory to verify the analytical results and define the biological integrity. Materials are stored and shipped from our Sittingbourne or Freiburg facilities.

Materials produced at our Sittingbourne facility are shipped directly to all our customers in all territories worldwide.

Antigens prepared at our Freiburg facility are supplied directly to clients in Europe and non-China Asia territories.  

For customers in America and China, our Freiburg antigens are distributed through our carefully selected and highly reputable partners.

Technical Documents

If you require any further product specific technical information or datasheets please submit your request to our technical team here

2054-diarect-immunodiagnostic-mar-2021-v1-thumbnail.jpg

Last updated: 26/03/2021

DIARECT Immunodiagnostic Components Product Brochure

Last updated: 25/06/2021

Clinical Chemistry Brochure

Last updated: 16/03/2022

CENP Antigens Brochure

Last updated: 16/03/2022

DFS70 Antigens Brochure

Last updated: 16/03/2022

Diabetes Antigens Brochure

Last updated: 16/03/2022

Glycoprotein 2 Antigen Brochure

Last updated: 16/03/2022

Histone, Nucleosome, dsDNA Antigens Brochure

Last updated: 16/03/2022

Intrinisc Factor Antigen Brochure

Last updated: 16/03/2022

MPO, PR3, BPI Antigens Brochure

Last updated: 16/03/2022

Myositis Antigens Brochure

Last updated: 16/03/2022

Nucleolin Antigens Brochure

Last updated: 16/03/2022

Ribosomal Phosphoproteins Brochure

Last updated: 16/03/2022

Primary Biliary Cirrhosis Antigens Brochure

Last updated: 26/03/2021

DIARECT Immunodiagnostic Components Product Brochure

Last updated: 25/06/2021

Clinical Chemistry Brochure

Last updated: 16/03/2022

CENP Antigens Brochure

Last updated: 16/03/2022

DFS70 Antigens Brochure

Last updated: 16/03/2022

Diabetes Antigens Brochure

Last updated: 16/03/2022

Glycoprotein 2 Antigen Brochure

Last updated: 16/03/2022

Histone, Nucleosome, dsDNA Antigens Brochure

Last updated: 16/03/2022

Intrinisc Factor Antigen Brochure

Last updated: 16/03/2022

MPO, PR3, BPI Antigens Brochure

Last updated: 16/03/2022

Myositis Antigens Brochure

Last updated: 16/03/2022

Nucleolin Antigens Brochure

Last updated: 16/03/2022

Ribosomal Phosphoproteins Brochure

Last updated: 16/03/2022

Primary Biliary Cirrhosis Antigens Brochure

There are currently no documents in this category.

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BBI Solutions Brands

DIARECT has a world leading recombinant autoimmune antigen capability immediately that enhances BBI’s portfolio and position as a ‘complete’ immunoassay reagent supplier.

Maine Biotechnology Services brings 28 years of knowledge, experience, process development, and project management to our customers to ensure the best possible antibodies for any application.

Novarum™ technology transforms a smartphone into a diagnostic test reader and work flow management system , empowering users to read and share the results of rapid tests at the point of care connecting patients and doctors, field workers, lab researchers and primary care clinicians to specialist practices as part of a mobile eco-system.

BioTeZ Berlin-Buch GmbH is a biotechnology company founded in 1992. BioTeZ is a partner of research institutions, the pharmaceutical industry and laboratory diagnostics.

STEFFENS BIOTECHNISCHE ANALYSEN GmbH has been developing and producing Enzyme-Linked Immuno Sorbent Assay (ELISA) test kits and components since 1991, both as an OEM manufacturer and for direct sales.

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Autoimmune and Inflammation

Autoimmune and inflammation biomarkers from BBI

Inflammation markers

Related Products

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Centromere Protein A (CENP-A)

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Alanyl-tRNA Synthetase (PL-12)

Alanyl-tRNA Synthetase (PL-12)

Glycyl-tRNA Synthetase (EJ)

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Asparaginyl-tRNA Synthetase (KS)

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